The Thermo Scientific Evolution One Plus UV-Vis Spectrophotometer is a versatile analytical instrument widely used for qualitative and quantitative analysis of samples by measuring the absorption of ultraviolet (190–400 nm) and visible (400–800 nm) light which offers variable spectral bandwidths (1.0 nm and 2.0 nm). It operates on the Beer-Lambert Law, where absorbance is proportional to concentration. Being an essential tool in environmental engineering for analyzing water pollutants, soil contamination, and molecular studies, making it ideal for environmental monitoring and research. Its rapid, reagent-free analysis ensures cost-effective and non-destructive testing. With applications in water treatment optimization and ecosystem monitoring, it plays a critical role in sustainable remediation strategies.