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Seminar on DNA-aptamer coupled DNA origami as bio-sensors and delivery vehicles by Banani Chakraborty, 18 January 2019

Date and Venue Information
18 January 2019, Friday, at 4 pm, Seminar Hall, Department of Chemistry

Title of Seminar
"DNA-aptamer coupled DNA origami as bio-sensors and delivery vehicles" by Professor Banani Chakraborty, Indian Institute of Science, Bangalore

Nucleic acid aptamers are selected to bind its target with high specificity; target can vary from small molecule, large proteins to cancer markers. Out of all the nucleic acid aptamers, DNA aptamers are very widely used for its facile chemical synthesis, simple handling and stability in wide spectra of working conditions in-vitro to in-vivo. DNA-aptamer based biosensors have found wide applications in sensing using various tools such as biochemical, electrochemical, fluorescent, plasmonics etc. To make the aptamers function in parallel and to detect multiple targets, immobilisation of multiple such aptamers are necessary. Here we use the latest success of DNA nanotechnology over last 3 decades which led to nano-meter scale surfaces named DNA origami. First origami was demonstrated in 2006 by Rothemund et.al. for the first time and now have become nano_architectural masterpiece of innumerable shapes and sizes using bottom up nanotechnology. We now focus on using some of the simpler DNA origami structures to functionalize them by immobilising sensors. Towards that vision, we started off by optimising the binding of multiple aptamers; using various fluorescence based techniques such as bulk Fluorescence Resonance Energy Transfer (FRET), Protein Induced Fluorescence Enhancement (PIFE) and single molecule FRET for small molecules such as Adenosine, Kanamycin and proteins such as Lysozyme, Thrombin etc. We are using well studied aptamers so that we can compare multiple techniques to come up with cheaper and more efficient ways to be translated from solution to DNA-origami platform. In summary, we aim to combine the precise point to point programmability of DNA origami nano surface and the target specificity of DNA aptamers to make detection of targets in parallel in a practical and cost effective manner. In future such sensing platforms can also be upgraded to deliver cargo in the desired location using various 3D shapes of DNA origami.
Brief Profile of the Speaker
Dr. Banani Chakraborty (Ph.D.) is Ramalingaswami  Fellow (DBT), Department of Chemical Engineering, IISc. Bangalore, INDIA. She has done is Ph.D from DNA Nanotechnology, New York University, USA in 2008.